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In contrast, although some of the ADAM12-transfected cells extended membrane protrusions, these protrusions were less robust and did not lead to effective cell body translocation (Movie 2B). As seen in Figure 7, mock-transfected cells migrated persistently at a rate of ∼10 μm/h. Twenty-four hours after transfection, cells were labeled with the membrane-impermeable EZ-Link NHS-LC-biotin (Pierce